Rapidly assaying cell viability for diverse bacteria species is not always straightforward. In eukaryotes, cell viability is often determined using colorimetric dyes; however, such dyes have not been identified for bacteria. We screened different dyes and found that erythrosin B (EB), a visibly red dye with fluorescent properties, functions as a vital dye for many Gram-positive and -negative bacteria. EB worked at a similar concentration for all bacteria studied and incubations were as short as 5 min. Given EB's spectral properties, diverse experimental approaches are possible to rapidly visualize and/or quantitate dead bacterial cells in a population. As the first broadly applicable colorimetric viability dye for bacteria, EB provides a cost-effective alternative for researchers in academia and industry.
Keywords: bacteria; cell viability; flow cytometry; membrane-exclusion dye; microscopy; vital dye.
Kim SI, Kim HJ, Lee HJ, Lee K, Hong D, Lim H, Cho K, Jung N, Yi YW. Kim SI, et al. Anal Biochem. 2016 Jan 1;492:8-12. doi: 10.1016/j.ab.2015.09.010. Epub 2015 Sep 21. Anal Biochem. 2016. PMID: 26399556
Krause AW, Carley WW, Webb WW. Krause AW, et al. J Histochem Cytochem. 1984 Oct;32(10):1084-90. doi: 10.1177/32.10.6090533. J Histochem Cytochem. 1984. PMID: 6090533
Herculano LS, Lukasievicz GV, Sehn E, Caetano W, Pellosi DS, Hioka N, Astrath NG, Malacarne LC. Herculano LS, et al. Appl Spectrosc. 2015 Jul;69(7):883-8. doi: 10.1366/15-07865. Epub 2015 Jun 1. Appl Spectrosc. 2015. PMID: 26036593
Sträuber H, Müller S. Sträuber H, et al. Cytometry A. 2010 Jul;77(7):623-34. doi: 10.1002/cyto.a.20920. Cytometry A. 2010. PMID: 20583280 Review.
Mudroňová D. Mudroňová D. Benef Microbes. 2015;6(5):727-34. doi: 10.3920/BM2014.0145. Epub 2015 Apr 22. Benef Microbes. 2015. PMID: 25869279 Review.
Abdelmajed MA, El-Din KMB, Attia TZ, Omar MA. Abdelmajed MA, et al. BMC Chem. 2024 Aug 27;18(1):158. doi: 10.1186/s13065-024-01261-9. BMC Chem. 2024. PMID: 39192355 Free PMC article.
Nguyen L, DeVico B, Mannan M, Chang M, Rada Santacruz C, Siragusa C, Everhart S, Fazen CH. Nguyen L, et al. Biomolecules. 2023 Sep 18;13(9):1404. doi: 10.3390/biom13091404. Biomolecules. 2023. PMID: 37759804 Free PMC article.
Trinh KTL, Lee NY. Trinh KTL, et al. Pathogens. 2022 Sep 16;11(9):1057. doi: 10.3390/pathogens11091057. Pathogens. 2022. PMID: 36145489 Free PMC article. Review.
Al-Madani H, Du H, Yao J, Peng H, Yao C, Jiang B, Wu A, Yang F. Al-Madani H, et al. Biosensors (Basel). 2022 Jun 24;12(7):453. doi: 10.3390/bios12070453. Biosensors (Basel). 2022. PMID: 35884256 Free PMC article. Review.
Wen J, Liu J, Wu J, He D. Wen J, et al. RSC Adv. 2022 Jan 11;12(3):1675-1681. doi: 10.1039/d1ra07287k. eCollection 2022 Jan 5. RSC Adv. 2022. PMID: 35425161 Free PMC article.